Journal: Bioactive Materials

Article Title: Modulating autophagic flux via ROS-responsive targeted micelles to restore neuronal proteostasis in Alzheimer's disease

doi: 10.1016/j.bioactmat.2021.09.017

Figure Lengend Snippet: Attenuation of Aβ burden and tau hyperphosphorylation in triple-transgenic mice by TT-NM/Rapa. (A) Quantification of the levels of soluble and insoluble Aβ 1-40 and Aβ 1-42 in 3 × Tg-AD mice treated with various rapamycin preparations or saline by ELISA kits. ( n = 5). (B) Representative immunohistochemical images of amyloid plaque deposit in the brain of 3 × Tg-AD mice treated with various rapamycin preparations or saline. Aβ plaque was stained with anti -β-Amyloid1-42 antibody. (C) Western blot analysis of p-tau proteins in the brain of 3 × Tg-AD mice treated with various rapamycin preparations or saline. ( n = 3). (D) Representative immunohistochemical images of neurofibrillary tangles in the cortex of 3 × Tg-AD mice treated with various rapamycin preparations or saline. Neurofibrillary tangles were stained with anti -Phospho-tau (Ser202, Thr205) antibody. (A) and (C) Data were presented as mean ± SD. # p < 0.05, ## p < 0.01, compared with AD group; * p < 0.05, ** p < 0.01, between the compared groups.

Article Snippet: Amyloid-β 1-40 Human ELISA Kit and Amyloid-β 1-42 Human ELISA Kit were purchased from Elabscience Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Transgenic Assay, Saline, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Staining, Western Blot

Journal: Cell Communication and Signaling : CCS

Article Title: BMAL1 ameliorates type 2 diabetes-induced cognitive impairment via AREG upregulation and PI3K/Akt/GSK-3β pathway activation

doi: 10.1186/s12964-024-02019-5

Figure Lengend Snippet: BMAL1 knockdown aggravated Alzheimer-like pathology. A , B Western blots and quantitation analysis of the phosphorylation levels of tau at Ser396, Thr231, and Ser199 ( n = 4 per group). C , D Western blots and quantitation analysis of the expression levels of APP and BACE1 ( n = 4 per group). E , F Immunohistochemical for Aβ plaques in the hippocampus of mouse and quantitation analysis in four groups (Scale bars = 400 µm in low-magnification and scale bars = 100 µm in high-magnification image) ( n = 3 per group). G ELISA of Aβ1-40 and Aβ1-42 in the hippocampus ( n = 4 per group). Data were plotted as means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. The data were analyzed by two-way ANOVA followed by post hoc Tukey’s HSD test

Article Snippet: The levels of Aβ1-40 and Aβ1-42 were measured using ELISA kits (E-EL-M3009 and E-EL-M3010, Elabscience) according to the manufacturer’s instructions.

Techniques: Knockdown, Western Blot, Quantitation Assay, Phospho-proteomics, Expressing, Immunohistochemical staining, Enzyme-linked Immunosorbent Assay

Journal: Cell Communication and Signaling : CCS

Article Title: BMAL1 ameliorates type 2 diabetes-induced cognitive impairment via AREG upregulation and PI3K/Akt/GSK-3β pathway activation

doi: 10.1186/s12964-024-02019-5

Figure Lengend Snippet: BMAL1 overexpression extenuated Alzheimer-like pathology. A , B Western blots and quantitation analysis of the phosphorylation levels of tau at Ser396, Thr231, and Ser199 ( n = 4 per group). C , D Western blots and quantitation analysis of the expression levels of APP and BACE1 ( n = 4 per group). E , F Immunohistochemical for Aβ plaques in the hippocampus of mouse and quantitation analysis in four groups (Scale bars = 400 µm in low-magnification and scale bars = 100 µm in high-magnification image) ( n = 3 per group). G ELISA of Aβ1-40 and Aβ1-42 in the hippocampus ( n = 4 per group). Data were plotted as means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. The data were analyzed by two-way ANOVA followed by post hoc Tukey’s HSD test

Article Snippet: The levels of Aβ1-40 and Aβ1-42 were measured using ELISA kits (E-EL-M3009 and E-EL-M3010, Elabscience) according to the manufacturer’s instructions.

Techniques: Over Expression, Western Blot, Quantitation Assay, Phospho-proteomics, Expressing, Immunohistochemical staining, Enzyme-linked Immunosorbent Assay

Journal: Cell Communication and Signaling : CCS

Article Title: BMAL1 ameliorates type 2 diabetes-induced cognitive impairment via AREG upregulation and PI3K/Akt/GSK-3β pathway activation

doi: 10.1186/s12964-024-02019-5

Figure Lengend Snippet: BMAL1 overexpression-induced cognitive improvement depends on PI3K/Akt/GSK-3β signaling pathway in db/db mice. A The experimental timeline. B , C The total distance traveled and average velocity was assessed in the open field test ( n = 8 per group). D , E The duration and frequency of exploring the novel object were assessed in the novel object recognition test ( n = 8 per group). F , G Acquisition errors and probe errors of the Barnes maze test were assessed for spatial learning ability ( n = 8 per group). H Representative track images of mice in the probe trial in the Barnes maze test. I Western blots of the phosphorylation levels of PI3K, Akt, and GSK-3β in the hippocampus ( n = 3 per group). J Western blots of the phosphorylation levels of tau at Ser396, Thr231, and Ser199 sites in the hippocampus ( n = 3 per group). K Western blots of the expression levels of APP and BACE1 in the hippocampus ( n = 3 per group). L Immunohistochemical for Aβ plaques in the hippocampus in four groups (Scale bars = 400 µm in low-magnification and scale bars = 100 µm in high-magnification image) ( n = 3 per group). M ELISA of Aβ1-40 and Aβ1-42 in the hippocampus ( n = 4 per group). Data were plotted as means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. The data were analyzed by two-way ANOVA followed by post hoc Tukey’s HSD test

Article Snippet: The levels of Aβ1-40 and Aβ1-42 were measured using ELISA kits (E-EL-M3009 and E-EL-M3010, Elabscience) according to the manufacturer’s instructions.

Techniques: Over Expression, Western Blot, Phospho-proteomics, Expressing, Immunohistochemical staining, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Protection against prolonged pneumococcal infection involves structural changes in C-reactive protein and subsequent binding to both phosphocholine and amyloids on the bacterial surface

doi: 10.3389/fimmu.2025.1631409

Figure Lengend Snippet: Detection of Aβ epitopes on the surface of S. pneumoniae . Microtiter wells were coated with pneumococci. Anti-Aβ antibodies, both monoclonal and polyclonal, were then added to the wells. Normal mouse IgG and rabbit IgG were included as controls. Bound monoclonal anti-Aβ antibodies and normal mouse IgG were detected by using HRP-conjugated goat anti-mouse IgG. Bound polyclonal anti-Aβ antibodies and normal rabbit IgG were detected by using HRP-conjugated donkey anti-rabbit IgG. The OD of the developed color was read at 405 nm. Data shown are mean ± SEM of three experiments.

Article Snippet: Both, polyclonal anti-Aβ antibodies (Novus, NBP2-25093) and monoclonal anti-Aβ antibodies (Novus, NBP2-13075) were used to detect the amyloids on the coated pneumococci.

Techniques: